Articular cartilage injuries have a limited potential to heal and, over time, may lead to osteoarthritis, an inflammatory and degenerative joint disease associated with activity-related pain, swelling, and impaired mobility. Regeneration and restoration of the joint tissue functionality remain unmet challenges. Stem cell-based tissue engineering is a promising paradigm to treat cartilage degeneration. Hydrogels have emerged as attractive biomaterials for this purpose, due to their biocompatibility and ability to mimic the tissue extracellular matrix. Recently, graphene oxide (GO) has emerged as new nanomaterial for cartilage tissue engineering due to chondroinductive properties when embedded into polymeric formulations. It has been also shown that piezoelectric nanomaterials, like barium titanate (BaTiO3) nanoparticles, can be exploited as nanoscale transducers capable of inducing cell growth/differentiation. Ultrasound waves are an interesting tool to facilitate chondrogenesis. In particular, it has been demonstrated that low-intensity pulsed ultrasound (LIPUS) regulates the differentiation of adipose mesenchymal stromal cells (ASCs). The aim of this study was to investigate whether dose-controlled LIPUS is able to influence chondrogenic differentiation of ASCs embedded in a 3D piezoelectric hydrogel.

LOW-INTENSITY PULSED ULTRASOUND INDUCES CHONDROGENIC DIFFERENTIATION OF ADIPOSE-STROMAL CELLS IN 3D PIEZOELECTRIC HYDROGEL

Cafarelli, A.;Ricotti, L.;
2022

Abstract

Articular cartilage injuries have a limited potential to heal and, over time, may lead to osteoarthritis, an inflammatory and degenerative joint disease associated with activity-related pain, swelling, and impaired mobility. Regeneration and restoration of the joint tissue functionality remain unmet challenges. Stem cell-based tissue engineering is a promising paradigm to treat cartilage degeneration. Hydrogels have emerged as attractive biomaterials for this purpose, due to their biocompatibility and ability to mimic the tissue extracellular matrix. Recently, graphene oxide (GO) has emerged as new nanomaterial for cartilage tissue engineering due to chondroinductive properties when embedded into polymeric formulations. It has been also shown that piezoelectric nanomaterials, like barium titanate (BaTiO3) nanoparticles, can be exploited as nanoscale transducers capable of inducing cell growth/differentiation. Ultrasound waves are an interesting tool to facilitate chondrogenesis. In particular, it has been demonstrated that low-intensity pulsed ultrasound (LIPUS) regulates the differentiation of adipose mesenchymal stromal cells (ASCs). The aim of this study was to investigate whether dose-controlled LIPUS is able to influence chondrogenic differentiation of ASCs embedded in a 3D piezoelectric hydrogel.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11382/546333
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