Devices and methods for multiplexing liquid in biosensor micro-chambers

A biosensors device for simultaneous multiple-analyte analysis by a plurality of biosensors dipped in single biosensor micro-chamber flooded with a functional liquid flowing to the biosensor micro-chambers from a fluidic circuit with no symmetry prerequisite comprises micro-valves operably associated to biosensor micro-chambers for selectively allowing the crossing of a functional liquid to the biosensor micro-chambers, a biosensor micro-chamber at a time, according to a pulsating flow mode so that the total flow rate can be greatly reduced and a predetermined amount of a pressured functional liquid is multiplexed to biosensor micro-chambers. Companion methods are defined for such a biosensors device.